| 05 Feb 2021 20:00 | Hertha BSC | 0 : 1 | Bayern |
| 05 Feb 2021 18:30 | Erzgebirge Aue | 3 : 3 | Hamburger SV | |
| 05 Feb 2021 18:30 | FC St. Pauli | 2 : 1 | SV Sandhausen |
| 05 Feb 2021 21:00 | Deportivo Alavés | 1 : 0 | Real Valladolid |
| 05 Feb 2021 20:45 | Fiorentina | 0 : 2 | Internazionale |
| 05 Feb 2021 18:00 | Rio Ave | 0 : 0 | CD Nacional | |
| 05 Feb 2021 20:00 | Marítimo | 0 : 2 | Sporting CP | |
| 05 Feb 2021 20:00 | SL Benfica | 0 : 0 | Vitória SC | |
| 05 Feb 2021 22:00 | Boavista | 1 : 2 | Gil Vicente FC | |
| 05 Feb 2021 22:00 | Paços de Ferreira | 2 : 1 | Tondela |
| 05 Feb 2021 20:00 | Fortuna Sittard | 0 : 1 | Heracles Almelo |
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