| 12 Feb 2021 20:30 | RB Leipzig | 2 : 1 | Augsburg |
| 12 Feb 2021 18:30 | Hannover 96 | 0 : 0 | SC Paderborn 07 | |
| 12 Feb 2021 18:30 | Holstein Kiel | 1 : 0 | FC Würzburger Kickers |
| 12 Feb 2021 21:00 | Celta Vigo | 3 : 1 | Elche |
| 12 Feb 2021 20:45 | Bologna | 1 : 1 | Benevento |
| 12 Feb 2021 21:30 | FC Famalicão | 0 : 0 | Belenenses SAD |
| 12 Feb 2021 18:45 | RKC Waalwijk | 1 : 0 | FC Emmen |
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